Sixisoforms of 14-3-3 proteins in the extracts of mouse oocytes versus eggs aredetected by the western blot technique which is shown below in the picture. Thedetection is done by 14-3-3 isoform-specific antibodies. The extracts of theovary and brain are kept as the positive controls. Separate genes encode the14-3-3 proteins which are a family of homologous, conserved and ubiquitouslyexpressed proteins. There are six mammalian isoforms of the 14-3-3 proteinwhich are denoted by Greek letters as represented below.
1. Which of the following statements is not true regarding thesix 14-3-3 protein isoforms in mouse oocytes versus eggs mentioned in theexperiment?A) All the sixisoforms of 14-3-3 proteins shown, are translated individually from differentmRNA transcripts in oocytes as well as eggsB) Amount of14-3-3 ? appears to be greater in oocytes compared to eggsC) Amount of14-3-3 ? appears to be lesser in oocytes compared to eggsD) Cellularamounts of all the six 14-3-3 isoforms do not remain unchanged duringmaturation of oocytes into eggs DNA,the genetic material is one of the most important types of biomolecules in alleukaryotic cells. DNA is packaged within the nucleus as chromosomes.Recombination occurs at the DNA strands in non-sister chromatids of homologouschromosomes. The breakage and rejoining of the two DNA strands are involved inthe recombination process.
This breakage and rejoining of the two DNA moleculesresults in the formation of two re-arranged strands. This leads to theintroduction of genetic variations in a population. The below process shows the process ofgenetic recombination at a single recombination site between two such DNAstrands, M and F and two new sequences are generated by the formation of C1 andC2 strands. All sequences are represented5′ to3′ direction.
9. In an experiment conducted to demonstrate the process ofrecombination, which of the following would be highlighted as in vivo biological events resultingrecombination of genetic material? A) Synapsis andcrossing over in mitosisB) Synapsis andcrossing over in meiosisC) Synaptictransmission D) Synaptonemalcomplex