Non of the whole animal. TPM made it possible

Non linear processes of light leads to develop different opticalmicroscopy in last two decades. Arif thesis.

Two photon microscopy TPM  is one of the microscopies developed on thebasis of non linear interaction of light. Arif et al reported that TPM hasalready achieved its effectiveness in ” biological imaging, especially in thicktissue and live animal studies”. In the past when linear effet of light was used for imaging of tissue itwas not possible to obtain clear image from a appreciable depth of tissue dueto strong scattering of light.  Non-linear microscopy is less sensitive toscattering which facilitates it to use in biological tissue imaging in highresolution 1. In present times TPM is prominent tool for imaging in manyscientific field because of its capability of reducing  photo damage, imaging of live tissue with highresolution from a appreciable depth.{important paperTwo photon excitation is a 2nd orderprocess where a molecule excited to the electronic state from its ground stateby absorbing two photons. The sum of the energies of the two photon iscomparable to the energy differences of the states.

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The rate of absorption isdependent on the square of the light intensity which makes it different fromlinear process.TPM made itpossible to investigate the dynamic of cellular process of living organism in anincreased depth of tissues but with less phototoxicity. With the introductionof TPM it become possible to address the exact biological and clinical concernof the whole animal. TPM made it possible to visualize clearly and with moredetails even the nearly transparent tissues.

So far, its reported that, Impactof T-cell in brain 3, lipid structure and cell domain 4, kidney dynamics 5have been visualized by TPM method. This method facilitates to improve themedical science to address the malfunctioning of organism more accurately.